To create a montage at each position do the following steps:
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In the Remote panel - choose the sample type - Slide.
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In the Expander panel, go to Protocol Manager and select Multi-Position Settings.
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Add the positions to be imaged.
4. Set the Multi-position order
You can change how the positions are visited according to time for the Protocol and if there should be a time delay between each position:
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At each time point visit all positions Visits all positions for each time point, moving to each successive time point.
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At each position run a time series Runs a time series at each position in the order shown in the table. A montage shall be run at each time point.
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Select the type of Montage
You can select either “Tiles” or “Perimeter”. See see https://oiandor.atlassian.net/wiki/x/ZQBD for further information.
5a. Tile Montage
Select "Montage Enabled" and specify the 'Tiles' montage.
5b. Perimeter Montage (Note this option is only available with a valid Irregular Montage license)
To create a perimeter-defined montage at each position do the following steps:
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Change the Montage Mode to Perimeter.
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Expand the Multi-Position settings and select one of the positions in the table.
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In the Montage section, press the button labelled “Draw Montage for Field“
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Click on the image to draw the perimeter of your montage. Press Enter or Right-click to auto-complete the perimeter.
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Repeat the process for each multi-position that requires a montage. Perimeter montages take their Z position from the related Multi-Field.
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For positions that do not have a perimeter montage specified, a single field is acquired.
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If you want to have the same irregular montage for each position, use the “Copy To All Fields” button. These can then be updated if required, after copying is complete.
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A confirmation message will appear:
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If you need to delete the irregular montages for every position, use the “Delete All Montages” button.
A confirmation message will appear:
Related content
Video tutorial can be found here: Multi Position Irregular Montage