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Video Tutorials

Andor Benchtop Confocal BC43 - Tutorials

Below is the complete set of video tutorials for BC43 split into hardware and software sections. Each video has a title, a brief description and the duration of the corresponding video.

Hardware

Overview of BC43: Overview and Switching on-off Software (3 min 19s)

https://youtu.be/9eNlxlKGlmg?si=haNgD4KiovELfdxr

Sample Holder (1min 57s)

https://youtu.be/0YbveM5S20s?si=hjjwpoYD--Bdnj8e

Sample Navigation (3mins 38s)

https://youtu.be/JfDBE-LY0iM?si=Zk4sGAwwzqV44zKt

Add and Cleaning Oil (5mins 2s)

https://youtu.be/Hx3OJ59PtIw?si=DrEtLuWJF5-MqBgC

Taking your first Image (4mins 56s)

https://youtu.be/dcKj8VVUVu8?si=ekgt2yNVFMkcHgKt

Using SuperResolution(2mins 29s)

https://youtu.be/CIuTdmQFJ5M?si=9Rdg4d_5pBaAFkdj

Creating a SuperResolution Protocol (2m17s)

https://youtu.be/h2Tw60UoZLM?si=Hf3I8vfaE3DXoaah

Getting the best from Super Resolution (3min 47s)

https://youtu.be/v68rvrxKp98?si=YJbvtAHhNbUfWaSI

Rooting the objective heater cable - How to root the objective heater cable (3 min)

Software

The Preferences tab - See where to save data images & other options (2 min)

Image Acquisition Tools: The Remote Panel (7mins 4s)

https://youtu.be/Q6P2obU2yP8?si=bTZCUTGGZ8WMIQz3

The Image Viewer Panel - Overview of image viewer panel tools and options to visualize the acquired data (15 min)

Image acquisition: Overview of Protocol Creation - Quick overview of the protocol manager options (4 min)

Image acquisition: Combine DPC with Confocal or Widefield - Combine transmitted light with Fluorescence (confocal or Widefield) (2 min)

Image acquisition: Acquire a Time Series Protocol - How to acquire a time series (3 min)

Image acquisition: Acquire a Z stack - How to acquire a Z stack using either the start and stop option or the set centre option (4 min)

Image acquisition: Acquire a Z scan (in Widefield) add Deconvolution - See how to acquire a Z scan in widefield mode and activate deconvolution in the protocol (2 min)

Image acquisition: combine Time-Series with Z-scan - Quickly see how to combine Z and time in a single acquisition (2 min)

Image acquisition: Acquire a Multi-Position protocol - How to acquire multiple positions (7 min)

Image acquisition: Combine Multi Position with Z (Z centre) - How to acquire multiple positions with Z. The Z scan is set by setting the centre of the image (5 min)

Image acquisition: Combine Multi Position with Z (Start -End) - How to acquire multiple positions with Z. The Z scan is set by setting the Start and Stop (7 min)

Image acquisition: Acquire a Montage (set perimeter) - How to acquire a montage by defining 2 points that set the perimeter of the image (2 min)

Image acquisition: Acquire a Montage (set tiles) - How to acquire a montage by setting the number of tiles around the chosen centre of the montage (4 min)

Image acquisition: Acquire a Multi-Well Protocol - How to image a Multi-Well plate (6 min)

Image acquisition: Open or import a protocol - How to re-use previously created protocols (1 min)

Image Processing Options- Deconvolution and Stitching - Overview of Options in Deconvolution and stitching (10 min)

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