Setting up and performing Photostimulation with Mosaic

To run a Photostimulation experiment using the Mosaic, you need to do the following:

1. Ensure that suitable Beamsplitter Plugs are installed in the Mosaic and a suitable microscope cube for the protocol is available: See Beamsplitter Plugs and Microscope Cubes for Photostimulation 

2. Create a Photostimulation mode and then specific imaging channels that will contain the illumination wavelength, power and duration information. If not already configured and available please see Creating Photostimulation Channels

3. Calibrate the Mosaic. The Mosaic needs to be calibrated so that the on screen image matches that of the DMD array, using a suitable calibration slide (see: Mosaic Setup and Calibration).

4. Then finally the protocol can be setup for your photostimulation experiment. Note that on initial setup, your Dragonfly system should have a selection of Photostimulation channels created, these can be edited if required to suit a specific experiment. The following instructions guide you through creation of a new photostimulation channel.Creating and Running a Photostimulation Protocol