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Through Series Analysis

Performing a Through-series Analysis

This topic shows how to use the Through-series Analysis functions.

In the current version of Fusion, ROIs (Regions of Interest) not supported but overlays are supported for Through-Series analysis.

  1. Navigate to the Analysis panel.

  2. Select Show through-series Analysis

  3. Select the required settings e.g. Time Series or Z-stack.

  4. Click Start Analysis

    1. You can draw overlays onto your image using Overlays.

  5. The graph is displayed:

    1. If your only have single channel selected then the chart will use the overlay colour otherwise it will use the channel colour.

Above: Through-series Analysis Graph view 

To view the data as a table with statistics, click the more button (bottom right). Use the scroll bars to scroll across through the statistics data.

 Above: Data table and Statistics view

Viewing Options

If you modify the overlay or settings, click Start Analysis to refresh the data if auto-analysis* is not enabled. There are a number of options for viewing the image data. You can change how the data is displayed in the following ways:

Display using Logarithmic scale

View the graph using a log scale.

Show a graph per channel

This shows a separate graph for each channel used in the image.

Show legend

This shows a legend beside the graph(s) for each channel.

Always Auto Range

Automatically scales the graph based on the data. When this is disabled you can use the mouse buttons to pan and zoom the scale and the graph plot. Auto Range can also be used in manual display mode to do a single-shot scale to fit data.

Max and Min

Use the Min and Max option buttons to plot the through-series for the max or min values of the series.

Background

Change the background colour for the graph from the palette and advanced colour options.

Filter Outlier Pixels

Use this option to filter pixels within the top and bottom 1% ranges.

Adjust Graph Axes

This button enables min and max values for the X and Y axes to be entered.

*With Auto Analysis if you change any setting then analysis will be stop immediately and restart 1 secs after your last interaction.

Exporting the Graph and Data

There are two options for exporting the graph from the Through-series Analysis graph. With the graph displayed the export buttons are located to the top right:

Export graph view to clipboard

Useful for pasting the graph into another document.

Export graph view to an image file

For saving the graph as an image file (jpg, png or bmp formats).

To export the data, click the more button to view the data as a table.

Copy to clipboard

Useful for pasting the data (and image metadata) into an other document e.g. Excel document

Export to file

For saving the data as a .csv file for use in other applications

Video Tutorials can be found here: Through Series Analysis

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