Fusion Dragonfly Software Guide
Fusion Dragonfly Software Guide
Breadcrumbs

Creating a Multi-Position - Montage

It is possible to create a montage at multiple positions. This can be done as follows:

  1. In the Remote panel - choose the sample type - Slide.

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  1. Go to Protocol Manager in the expanded panel and select Multi-Position Settings.

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  1. Add the positions to be imaged.

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  1. Set the Multi-position order

You can change how the positions are visited according to time for the Protocol and if there should be a time delay between each position:

  1. At each time point visit all positions   Visits all positions for each time point, moving to each successive time point.

  2. At each position run a time series       Runs a time series at each position in the order shown in the table. A montage shall be run at each time point.

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  1. Select the type of Montage

You can select either “Tiles” or “Perimeter”. See Creating a Montage for further information.

5a. Tile Montage

Select "Montage Enabled" and specify the 'Tiles' montage.

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5b. Perimeter Montage (Note this option is only available with a valid Irregular Montage license)

To create a perimeter-defined montage at each position do the following steps:

  1. Change the Montage Mode to Perimeter.

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  1. Expand the Multi-Position settings and select one of the positions in the table.

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  1. In the Montage section, press the button labeled “Draw Montage for Field“

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  1. Click on the image to draw the perimeter of your montage. Press Enter or Right-click to auto-complete the perimeter.

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  1. Repeat the process for each multi-position that requires a montage. Perimeter montages take their Z position from the related Multi-Field.

    1. For positions that do not have a perimeter montage specified, a single field is acquired.

    2. If you want to have the same irregular montage for each position, use the “Copy To All Fields” button. These can be updated separately after copying is complete

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A confirmation message will appear:

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  1. If deleting the irregular montages for every position, use the “Delete All Montages” button.

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A confirmation message will appear:

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Note:

Important reminder for Leica DMi8 and Olympus ZDC

If the user decides to use the focus drift correction on a Multi-Well or a Multi-Field Multi-Well experiment. The Drift stabilisation must be activated for each well (or position).


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Go to the following pages for more information on:

Creating a Multi-Position Protocol

Creating a Montage